实验记录本

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1. T7 mutant structure analysis目的：分析T7突变体在yeast中转录效果提高的结构基础。 内容：以PDB 1H38 WT T7结构为基础，标出Alper使用的T7突变体突变位点，标出厉思宏进化T7突变体突变位点（1H38氨基酸加12等于厉思宏氨基酸位点）。 2022-07-31分析软件Chimera，原始文件1H38，T7蛋白的结构域包括 N-terminal domain (1-325)负责启动子识别和DNA打开 灰色标出，thumb sub-domain (residues 326–411)是催化结构的一部分 紫色标出，palm sub-domain, containing residues 412–553 and 785–879主要催化中心 蓝色标出，fingers sub-domain (aa 554–784)是催化结构的一部分 绿色标出，The residues 740-769 form a specificity loop负责启动子识别 红色标出。此外DNA序列 黄色标出，RNA序列 粉色标出，序列的5’端 红色标出。our mut 代表厉思 ...

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